gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2013)

22.10. - 25.10.2013, Berlin

A lipidomic study of phospholipid classes and species in human synovial fluid

Meeting Abstract

  • presenting/speaker Marta Kosinska - Universität Gießen, Orthopädische Universitätsklinik, Labor für Experimentelle Orthopädie, Gießen, Germany
  • Gerhard Liebisch - Universitätsklinik Regensburg, Institut für Klinische Chemie und Laboratoriumsmedizin, Regensburg, Germany
  • Günter Lochnit - Universität Gießen, Biochemisches Institut des FB Medizin, Gießen, Germany
  • Jochen Wilhelm - Universität Giessen, Medizinische Klinik II/IV, Gießen, Germany
  • Heiko Klein - Universität Gießen, Orthopädische Universitätsklinik, Labor für Experimentelle Orthopädie, Gießen, Germany
  • Markus Rickert - Universität Gießen, Orthopädische Universitätsklinik, Labor für Experimentelle Orthopädie, Gießen, Germany
  • Gerd Schmitz - Universitätsklinik Regensburg, Institut für Klinische Chemie und Laboratoriumsmedizin, Regensburg, Germany
  • Juergen Steinmeyer - Universität Gießen, Orthopädische Universitätsklinik, Labor für Experimentelle Orthopädie, Gießen, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2013). Berlin, 22.-25.10.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. DocPO13-982

doi: 10.3205/13dkou619, urn:nbn:de:0183-13dkou6195

Veröffentlicht: 23. Oktober 2013

© 2013 Kosinska et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Membrane phospholipid (PL) species contribute to boundary lubrication that is provided by synovial fluid (SF). Altered levels of lubricants such as lubricin, hyaluronan and PL are associated with increased friction, leading to articular cartilage damage. The aim of our lipidomic study was to determine whether the composition of PL species correlates with diseases of human knee joints using SF from non-affected controls, early osteoarthritis (eOA), late OA, and rheumatoid arthritis (RA) patients.

Methods: Lipids were extracted from cell-, and vesicle-free SF of 9 post-mortem donors (control) and 17 eOA, 13 late OA, and 18 RA patients. PL classes and species were quantified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Subsequently, the quantitative values of all PL species were corrected for possible dilution of SF according to the method described by Kraus et al (2002). This study was approved by the local ethics committee of Justus-Liebig-University Giessen, and all patients provided written informed consent. Statistically significant differences in PL concentrations between cohorts were analyzed by Kruskal-Wallis test. Adjustments for multiple testing in the series of Kruskal-Wallis tests were made to control the false discovery rate (FDR), which was set to 10%. Subsequently, the statistical significances of pair wise differences in median concentrations of PL species between cohorts were analyzed by Wilcoxon rank-sum test. P values of less than 0.05 were considered statistically significant.

Results and conclusion: By lipidomic analysis, we have provided the first detailed overview of PL species in human SF. We identified 130 lipid species belonging to 8 lipid classes (phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, plasmalogens, phosphatidylserine, phosphatidylglycerol, sphingomyelin, and ceramides). Compared to control SF, eOA and late OA had higher levels of most PL species. Moreover, the concentrations of 64 and 27 PLs modulated between RA and eOA respectively late OA SF. The levels of 66 PL species were significantly altered in eOA SF versus late OA.

Disease- and stage-dependent differences of PL species in SF alter articular joint lubrication. Increased levels of PLs during OA may constitute a response to enhance articular surface lubrication. Because certain PLs scavenge reactive oxygen species (ROS) and are pro- or anti-inflammatory, any altered PL level might influence ROS-scavenging activity of SF and the inflammatory status of joints. Thus, PLs mediate in part the pathogenesis of OA.