Artikel
Selenium – A Novel Radiosensitizer?
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Veröffentlicht: | 20. März 2006 |
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Gliederung
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Background: A radioprotective effect of low selenium doses on normal tissue and a possible radiosensitization of tumor cells at higher concentrations have been previously suggested. However, systematic experimental and clinical data are scarce.
Materials and Methods: C6 cells were cultured for 14 days at different selenite concentrations (0 µM, 2 µM, 3 µM and 3.6 µM) and irradiated with 0 Gy to 20 Gy. Plating efficiency and survival were determined using the colony assay.
Results: The overall radiosensitivity was low (D0,control=6.1 Gy). Irradiated cells exposed to increasing selenite concentrations showed a lower plating efficiency and, for doses > 2 Gy, a lower survival than the control. For Se concentrations of 0 µM, 2µM and 3 µM, respectively, SF2 amounted to 0.72, 0.48 and 0.46, and SF5 to 0.37, 0.25 and 0.12. Student’s t-test yielded a significant difference between the 0 µM and 3 µM curves at 5 Gy (p=0.02) and 10 Gy (p=0.009).
Conclusion: Our experiments suggest a radiosensitizing effect of selenite in glioma cells at concentrations of 2-3 µM.