gms | German Medical Science

49. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC), 42. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen e. V. (DGPRÄC), 16. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen e. V. (VDÄPC)

29.09. - 01.10.2011, Innsbruck

Optimization of tissue engineering conditions for bone formation in a vascular pedicle model

Meeting Abstract

  • author Holger Engel - BG-Unfallklinik Ludwighafen, Plastische Chirurgie
  • J.J. Huang - Division of Reconstructive Microsurgery, Dep. of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan
  • B. Yang - Division of Reconstructive Microsurgery, Dep. of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan
  • S.W. Kao - Division of Reconstructive Microsurgery, Dep. of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan
  • C. Yang - Division of Reconstructive Microsurgery, Dep. of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan
  • B. Jiang - Pritzker Institute of Medical Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL
  • E.M. Brey - Pritzker Institute of Medical Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL
  • M.H. Cheng - Division of Reconstructive Microsurgery; Dep. of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan
  • M. Lehnhardt - Dep. of Hand, Plastic & Reconstructive Surgery, -Burn Center-, BG Trauma Center, Ruprecht-Karls University of Heidelberg, Ludwigshafen, Germany

Österreichische Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie. Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 49. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC), 42. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 16. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Innsbruck, 29.09.-01.10.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgpraecP98

DOI: 10.3205/11dgpraec282, URN: urn:nbn:de:0183-11dgpraec2821

Veröffentlicht: 27. September 2011

© 2011 Engel et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: The repair of large bone defects following trauma, infection and tumor resection remains a major clinical challenge. Theoretically, bone tissue engineering could solve the problem of limited donor tissue availability without donor site morbidity. Prefabrication approaches developed in the field of reconstructive microsurgery can be exploited to enhance neovascularization.in engineered tissues. A particularly promising approach is to implant materials around a vascular pedicle that allows de novo vascularization While progress has been made in this approach, our incomplete knowledge about the role of different vascular components influencing neovascularization, the correlation between osteoinductive periosteum and the optimal combination of stem cells and target cells limits the ability for further progress. This study was carried out to evaluate the role of different vascular components inside a tissue engineering chamber, the role of periosteum as an osteoinductive factor and the impact of different compounds of stem cells and osteocytes as target cells.

Materials and methods: Sprague-Dawley rats were used for a pedicled groin fat flap based on the inferior epigastric vessels. The pedicles, femoral artery and vein, were isolated and employed as vascular carriers inside a silicone tube as the tissue engineering chamber. PEG-PLLA was used as scaffold mixed with different amounts of bmMSC and osteocytes. The fat groin flap was wrapped around the silicone chamber. At different time points (3d, 1w, 3w and 12w) the TE chamber was harvested and histologic and molecular analysis, blood vessel density, immunohistochemistry and quantification of VEGF was performed.

Results: Gene expression with vein and A+V seemed to be superior in terms of VEGF, TGFß and HGF expression. Periosteum is a critical factor as an osteoinductive component inside a TE chamber.