Artikel
The use of human sweat gland-derived stem cells for enhancing vascularization during dermal regeneration
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Veröffentlicht: | 27. September 2011 |
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Gliederung
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Vascularization is a key process in tissue engineering and regeneration and represents one of the most important issues in the field of regenerative medicine. Thus, several strategies to improve vascularization are currently under clinical evaluation. In the present study, stem cells derived from human sweat glands were isolated, characterized, seeded in collagen scaffold, and engrafted in a mouse full skin defect model for dermal regeneration. Results showed that these cells exhibit high proliferation rates and express stem and differentiation markers. Moreover, cells responded to angiogenic environments by increasing their migration (p<0.001) and proliferation (p<0.05) capacity and forming capillary like structures in matrigel. After seeding in the scaffolds, cells distributed homogeneously, interacting directly with the scaffold and released bioactive molecules involved in angiogenesis, immune response and tissue remodeling. Metabolic assays showed that cells were viable for at least 2 weeks in the scaffolds in vitro. In vivo, scaffolds containing cells were used to induce dermal regeneration in a full skin dermal regeneration model. Here we have found that the presence of human sweat gland-derived stem cells significantly improved dermal neovascularization (p<0.001). Since autologous sweat gland-derived stem cells are easy to obtain, exhibit a good proliferation capacity and improve vascularization during dermal regeneration, we suggest that the combined use of sweat gland-derived stem cells and scaffolds for dermal regeneration might be used to improve dermal regeneration in future clinical settings.