Artikel
Single cell derived vascular resident endothelial progenitor cells enhance vascularization in Scaffold-based skin regeneration
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Veröffentlicht: | 27. September 2011 |
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Gliederung
Text
Increasing evidence suggest that vascular resident endothelial progenitor cells (VR-EPCs) are present in several organs, playing an important role in postnatal neovascularization. Here, we isolated and characterized VR-EPCs from cardiac tissue in vitro, evaluating their regenerative potential in vivo. VR-EPCs showed to be highly clonogenic and expressed several stem and differentiation markers. Under endothelial differentiation conditions, cells form capillary-like structures, in contrast to osteogenic or adipogenic differentiation conditions where no functional changes were observed. After seeding in scaffolds, cells were distributed homogeneously and directly attached to the scaffold. Then, cell seeded scaffolds were used to induce dermal regeneration in a nude mice full skin defect model. The presence of VR-EPCs enhanced dermal vascularization. Histological assays showed increased vessel number (p<0.05) and cellularization (p<0.05) in VR-EPCs group. In order to explore possible mechanisms of vascular regeneration, in vitro experiments were performed. Results showed that proangiogenic environments increased the migration capacity (p<0.001) and ability to form capillary-like structures (p<0.05) of VR-EPC. In addition, VR-EPCs secreted severalpro-angiogenic molecules including VEGF and PDGF. These results indicate that a highly clonogenic therapeutic population of VR-EPCs might be established in vitro, representing a new source for therapeutic vascularization in tissue engineering and regeneration.