gms | German Medical Science

65. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

11. - 14. Mai 2014, Dresden

Time-dependent effects of ethyl pyruvate on inflammation and histological damage after controlled cortical impact in rats

Meeting Abstract

  • Daniel Jussen - Klinik für Neurochirurgie, Dr. Horst Schmidt Kliniken, Wiesbaden
  • Nina Wenda - Institut für Neurochirurgische Pathophysiologie, Universitätsmedizin der Johannes Gutenberg Universität, Mainz
  • Peter Horn - Klinik für Neurochirurgie, Dr. Horst Schmidt Kliniken, Wiesbaden
  • Oliver Kempski - Institut für Neurochirurgische Pathophysiologie, Universitätsmedizin der Johannes Gutenberg Universität, Mainz
  • Faramarz Dehghani - Abteilung für Anatomie und Zellbiologie, Martin Luther Universität Halle-Wittenberg, Halle (Saale)
  • Beat Alessandri - Institut für Neurochirurgische Pathophysiologie, Universitätsmedizin der Johannes Gutenberg Universität, Mainz

Deutsche Gesellschaft für Neurochirurgie. 65. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC). Dresden, 11.-14.05.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. DocP 112

doi: 10.3205/14dgnc508, urn:nbn:de:0183-14dgnc5089

Veröffentlicht: 13. Mai 2014

© 2014 Jussen et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Inflammation plays a crucial role in secondary brain damage. Ethyl pyruvate (EP) is known to suppress inflammation after systemic infection, hemorrhagic shock, cerebral ischemia and traumatic brain injury. Because of the promising short-term effects our study was designed to investigate the time-dependent course of mechanisms of action of EP and the long-term effects after repeated administration on inflammation and histological damage after controlled cortical impact (CCI) in rats.

Method: We induced CCI (4 m/s, 2 mm, 200 ms) in male Sprague Dawley Rats. Animals were randomized to following groups (1) Sham (no CCI), (2) CCI + vehicle or (3) CCI + 75 mg/kg EP. EP was administered 30 min after CCI and further on daily. To investigate time-dependent mechanisms of action animals were sacrificed 1h, 3h, 6h, 18h, 24h, 3 days and 14 days after injury. Lesion volume was determined in hematoxylin and eosin stained brains, inflammation was determined via immunohistochemical determination of iba, GFAP and Interleukin-1β.

Results: We could show a time-dependent effect of EP on inflammation after CCI. In long-term survival there was a tendential reduction of lesion volume (vehicle: 9,18 ± 1,17 mm3 vs. EP 75 mg/kg: 7,31 ± 0,64 mm3). Furthermore, EP down-regulated microglial activation and Interleukin-1β release while activating astrocytes.

Conclusions: EP leads to a time-dependent modulation of inflammation. Long-term administration of EP after CCI tendentially decreases lesion volume with simultaneous reduction of microglial activation und Interleukin-1β release.