gms | German Medical Science

63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

13. - 16. Juni 2012, Leipzig

5-ALA fluorescence in native pituitary adenoma cell suspensions: basis for resection control and photodynamic therapy (PDT)?

Meeting Abstract

  • C. Ewelt - Klinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Deutschland
  • R. Reeker - Klinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Deutschland
  • H. Ardon - Klinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Deutschland; Katholieke Universiteit Leuven, Leuven, Belgium; Sint Elisabeth Ziekenhuis, Tilburg, Netherlands
  • H.J. Steiger - Klinik für Neurochirurgie, Heinrich-Heine-Universität, Düsseldorf, Deutschland
  • D. Hänggi - Klinik für Neurochirurgie, Heinrich-Heine-Universität, Düsseldorf, Deutschland
  • D. Prevedello - Department of Neurological Surgery, Ohio State University, Columbus, United States
  • B. Fischer - Klinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Deutschland
  • W. Stummer - Klinik für Neurochirurgie, Westfälische Wilhelms-Universität, Münster, Deutschland

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocP 095

DOI: 10.3205/12dgnc482, URN: urn:nbn:de:0183-12dgnc4829

Veröffentlicht: 4. Juni 2012

© 2012 Ewelt et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Aminolevulinic acid (5-ALA) may represent a diagnostic intra-operative tool and treatment option as photosensitizer for recurrent or subtotally operated pituitary adenomas. We used cell suspensions of native pituitary adenomas and immortalized pituitary adenoma cell line GH3 in order to provide evidence for 5-ALA induced intracellular porphyrin accumulation and for acute effects of the photodynamic therapy (PDT) in these cells.

Methods: Cell suspensions of pituitary adenomas in primary cell culture, immortalized pituitary adenoma cell line GH3 and glioma cell line U373 were incubated for 4 h in 50 and 100 μg/ml ALA in 5% CO2 in room air. 5-ALA fluorescence accumulation was measured by fluorescence activated cell sorting (FACS) after 4/6/8/10/12/18/33/40 hours. Further, subsequent irradiation using a diode laser (lambda = 635 nm, 40 mW/cm2, total fluence 25 J/cm2) was performed for 5-ALA-incubated cell suspensions of GH3 cell line and U373 cell line and three control groups ("laser only", "ALA only", "no drug no light"). Cell death was analyzed by colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability and cytotoxicity. Furthermore, clinical and histopathological features were correlated to our experimental findings.

Results: Incubation with 5-ALA in 50 and 100 μg/ml concentration revealed the strongest fluorescence accumulation in ten pituitary adenoma cell suspensions of 80% after 8 - 12 hours vs. 96% in the immortalized pituitary adenoma cell line GH3 after 33–40 hours and 91 % in the control glioma cell line U373 after 4-6 hours. In first trials, ALA incubation in 5% CO2 with subsequent PDT resulted in significant cell death of more than 70% and growth arrest in the immortalized adenoma cell line and the glioma cell line as well. The rate of cell death in all control groups was negligible.

Conclusions: Pituitary adenoma cell suspensions show porphyrin fluorescence after incubation with ALA. Furthermore, PDT of experimental pituitary adenoma cell suspensions resulted in significant cell death after irradiation. Therefore, 5-ALA may offer a diagnostic intra-operative tool for resection control and further as treatment option for recurrent and not completely resectable pituitary adenomas in-vivo.