gms | German Medical Science

63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

13. - 16. Juni 2012, Leipzig

Expression of aquaporin 5 and AQP5 polymorphism A(-1364)C is associated with peritumoral edema in meningiomas

Meeting Abstract

  • N. Lambertz - Abteilung für Neurochirurgie, Klinikum der Universität Duisburg-Essen, Essen
  • N.E. Hindy - Abteilung für Neurochirurgie, Klinikum der Universität Duisburg-Essen, Essen
  • A. Bankfalvi - Institut für Pathologie und Neuropathologie, Klinikum der Universität Duisburg-Essen, Essen
  • Y. Zhu - Abteilung für Neurochirurgie, Klinikum der Universität Duisburg-Essen, Essen
  • U. Sure - Abteilung für Neurochirurgie, Klinikum der Universität Duisburg-Essen, Essen
  • I.E. Sandalcioglu - Abteilung für Neurochirurgie, Klinikum der Universität Duisburg-Essen, Essen

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocP 072

DOI: 10.3205/12dgnc459, URN: urn:nbn:de:0183-12dgnc4590

Veröffentlicht: 4. Juni 2012

© 2012 Lambertz et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Aquaporins (AQP) are well known water-channel proteins; in particular AQP4 is known to be involved in the development and resorption of brain edema. The functional impact of further AQPs on brain edema remains unclear. We recently demonstrated an association of the AQP5 Polymorphism A(-1364)C and the severity of peritumoral edema in meningiomas. Currently we analyzed the correlation between AQP5 expression and peritumoral edema in meningiomas. Furthermore we investigated the association of the above mentioned single nucleotide polymorphism (SNP) and the AQP5 expression.

Methods: 89 patients suffering from meningioma were studied. Peritumoral edema was classified in three degrees based on the preoperative imaging and current literature. DNA was extracted from paraffin-embedded tumor tissue. For the immunohistochemistry routinely formalin-fixed, paraffin-embedded tumor tissue was processed, cut and mounted on slides followed by immunostaining with anti-AQP5 antibodies. AQP5 expression was scored positive, if at least 25 % of tumor cells showed any staining. We divided these patients into three subgroups with mild, moderate and strong AQP5 expression. Comparison of clinical variables and immunohistochemistry between genotypes was done using Student's t-test for continuous variables and Pearson's X2-test for categorical data using the Graph-Pad-Prism Software Version 4.0 and the SPSS-Software Version 17.0 (SPSS-Software, USA).

Results: 32.3% patients showed none, 15.4% mild, 29.2% moderate and 23.1% strong AQP5 expression. A significant association of peritumoral edema and expression of AQP5 could be detected (p=0.040). The genotype distribution strongly correlates with the expression of AQP5 (p=0.028): 73.3% of the patients with strong AQP5 expression had an AA genotype while only 20% of the patients with a mild expression of AQP5 were A-homozygote in the A(-1364)C genotype.

Conclusions: The occurrence and severity of peritumoral edema in meningiomas is correlated with the expression of AQP5. The AQP5 Polymorphism A(-1364)C goes along with less AQP5 expression. Taking into consideration our previous results which confirmed the association of the AQP5 SNP and the severity of brain edema, our current findings emphasize that AQP5 seems to play an important role in the development of peritumoral edema in meningiomas.