Artikel
Multi-spectral opto-acoustic tomography for three-dimensional in vivo imaging in experimental SAH in mice
Suche in Medline nach
Autoren
Veröffentlicht: | 4. Juni 2012 |
---|
Gliederung
Text
Objective: Repetitive non-invasive cerebral imaging in mice is still challenging due to small body / brain size and limited overall availability of advanced technical equipment. In particular, addressing disease models with ischemic pathophysiological episodes, measuring tissue oxygenation is of utmost interest but remains highly sophisticated. Thus, we applied the newly developed imaging modality of in vivo multi-spectral opto-acoustic tomography (MSOT) to mice, which underwent experimental subarachnoid hemorrhage (SAH).
Methods: Using hairless mice (n = 10), hemorrhage was induced by the intraluminal Circle of Willis perforation (CWp) model. For three-dimensional brain imaging, 24 h after induction of experimental SAH, MSOT was performed in vivo under anesthesia. Immediately after MSOT, the animals were euthanized, the skulls were frozen and histological slices were performed coronally every 0.5 mm.
Results: MSOT offered non-invasive real-time imaging of the murine brain and the surrounding SAH. The hemorrhages were visualized in the wavelength range of 700–880 nm and showed good correlation to histological slices.
Conclusions: MSOT represents a new and innovative technique for fast volumetric in vivo imaging, which delivers good spectrally-enriched optical contrast combined with high spatial resolution not affected by light scattering in deep tissues. Its potential for experimental research with respect to repetitive non-invasive detection of brain tissue hypoxia has to be further evaluated.