Artikel
Expression of neuronal migratory phenotype marker – doublecortin correlates with motility characteristics of normal and transformed astrocytic cell lines and human glial tumors
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Veröffentlicht: | 28. April 2011 |
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Gliederung
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Objective: to assess the expression of doublecortin (DCx), a protein necessary for neuronal migration in normal, transformed and neoplastic glial cell lines and possible presence of a specific subpopulation of DCx-positive cells in highly motile human glial tumors.
Methods: Fetal human astrocytes line (NHA), transformed cell lines (h-TERT/E6/E7; h-TERT/E6/E7/RAS; h-TERT/E6/E7/RAS/EGFR wt; h-TERT/E6/E7/RAS/AKT and h-TERT/E6/E7/RAS/Beta-3) developed in Department of Pathology, UVHSC and 2 established glioblastoma cell lines (U1242MG, U251MG) were used. 19 samples of glioblastomas were obtained from BTRC tissue bank, UCSF. 7 samples of low-grade astrocytomas were obtained from pathology tissue bank, UVHSC. Western blot analysis, motility analysis and immunohistochemistry for doublecortin (DCx) were performed as previously described (1-3).
- 1.
- I.M. Hussaini, et al. Glia 25, 71-84 (1999);
- 2.
- S. Mizumatsu, et al. Cancer Res 63, 4021-4027 (2003);
- 3.
- T. Hirose, et al. Cancer 79, 989-1003 (1997).
Results: All analyzed cell lines expressed DCx albeit at different levels. Expression levels varied from 6325 AU for NHA to 1909 AU for U251MG measured by relative densitometry. Importantly, DCx expression positively correlated with cellular motility in Neuromatrix for most of cell lines studied. Subsequent analysis of primary astrocytic tumors revealed presence of distinct subpopulations of DCx-positive cells in pilocytic astrocytomas and glioblastomas. In glioblastoma sections random, diffuse staining of scattered cells was present. The cytoarchitecture of these cells varied from bipolar, multipolar with conspicuous processes to polygonal cell with and without cytoplasmic processes.
Conclusions: Specific subpopulations of cells expressing DCx within glial tumors might be involved in a long distance migration from primary tumor site.