gms | German Medical Science

62. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgen (PNCH)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

07. - 11. Mai 2011, Hamburg

Expression of neuronal migratory phenotype marker – doublecortin correlates with motility characteristics of normal and transformed astrocytic cell lines and human glial tumors

Meeting Abstract

  • R. Rola - Department of Neurological Surgery, Medical University of Lublin, Lublin, Poland; Department of Physiopathology, Institute of Agricultural Medicine, Lublin, Poland; Brain and Spinal Cord Injury Center, Department of Neurological Surgery, UCSF, San Francisco CA, USA
  • S.R. VandenBerg - Brain and Spinal Cord Injury Center, Department of Neurological Surgery, UCSF, San Francisco CA, USA; Department of Pathology, UCSF, San Francisco, USA
  • I.M. Hussaini - Department of Pathology, University of Virginia Health Science Center, Charlottesville, USA
  • D. Morhardt - Brain and Spinal Cord Injury Center, Department of Neurological Surgery, UCSF, San Francisco CA, USA
  • T. Trojanowski - Department of Neurological Surgery, Medical University of Lublin, Lublin, Poland
  • J.R. Fike - Brain and Spinal Cord Injury Center, Department of Neurological Surgery, UCSF, San Francisco CA, USA

Deutsche Gesellschaft für Neurochirurgie. Polnische Gesellschaft für Neurochirurgen. 62. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgen (PNCH). Hamburg, 07.-11.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. DocP 090

DOI: 10.3205/11dgnc311, URN: urn:nbn:de:0183-11dgnc3118

Veröffentlicht: 28. April 2011

© 2011 Rola et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Objective: to assess the expression of doublecortin (DCx), a protein necessary for neuronal migration in normal, transformed and neoplastic glial cell lines and possible presence of a specific subpopulation of DCx-positive cells in highly motile human glial tumors.

Methods: Fetal human astrocytes line (NHA), transformed cell lines (h-TERT/E6/E7; h-TERT/E6/E7/RAS; h-TERT/E6/E7/RAS/EGFR wt; h-TERT/E6/E7/RAS/AKT and h-TERT/E6/E7/RAS/Beta-3) developed in Department of Pathology, UVHSC and 2 established glioblastoma cell lines (U1242MG, U251MG) were used. 19 samples of glioblastomas were obtained from BTRC tissue bank, UCSF. 7 samples of low-grade astrocytomas were obtained from pathology tissue bank, UVHSC. Western blot analysis, motility analysis and immunohistochemistry for doublecortin (DCx) were performed as previously described (1-3).

1.
I.M. Hussaini, et al. Glia 25, 71-84 (1999);
2.
S. Mizumatsu, et al. Cancer Res 63, 4021-4027 (2003);
3.
T. Hirose, et al. Cancer 79, 989-1003 (1997).

Results: All analyzed cell lines expressed DCx albeit at different levels. Expression levels varied from 6325 AU for NHA to 1909 AU for U251MG measured by relative densitometry. Importantly, DCx expression positively correlated with cellular motility in Neuromatrix for most of cell lines studied. Subsequent analysis of primary astrocytic tumors revealed presence of distinct subpopulations of DCx-positive cells in pilocytic astrocytomas and glioblastomas. In glioblastoma sections random, diffuse staining of scattered cells was present. The cytoarchitecture of these cells varied from bipolar, multipolar with conspicuous processes to polygonal cell with and without cytoplasmic processes.

Conclusions: Specific subpopulations of cells expressing DCx within glial tumors might be involved in a long distance migration from primary tumor site.