gms | German Medical Science

61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010
Joint Meeting mit der Brasilianischen Gesellschaft für Neurochirurgie am 20. September 2010

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21. - 25.09.2010, Mannheim

Neutrophils commonly penetrate the glioblastoma venule wall

Meeting Abstract

Suche in Medline nach

  • Richard E. Kast - Department of Psychiatry University of Vermont, Burlington, VT, USA
  • Marc-Eric Halatsch - Klinik für Neurochirurgie, Universitätsklinikum Ulm, Germany

Deutsche Gesellschaft für Neurochirurgie. 61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010. Mannheim, 21.-25.09.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP1714

DOI: 10.3205/10dgnc185, URN: urn:nbn:de:0183-10dgnc1855

Veröffentlicht: 16. September 2010

© 2010 Kast et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Desbaillets et al in 1997, and Fossati et al. in 1999 and Iwatsuki et al. in 2000 showed neutrophil infiltration in glioblastomas. We wanted to confirm these observations to better understand the role of neutrophils in glioblastoma growth.

Methods: Seven glioblastoma biopsies were dually stained with H & E and an esterase stain that preferentially stains neutrophils. The entire slide was examined for neutrophils by light microscopy and micrographs were taken with a CCD camera. Areas of necrosis were avoided and not scored.

Results: Thirty-five percent of the venules had neutrophils either adherant to venule luminal wall or in various stages of penetration of the wall. Of these venules with neutrophils, intramural degranulation was common and in 11% of these venules degranulation was seen within the vessel lumen.

Conclusions: We confirm the presence of neutrophils within glioblastoma tissue but could not confirm Fossati et al. and Iwatsuki et al.'s observation of neutrophils preferentially in areas of penetration into normal brain. We saw neutrophils preferentially penetrating venules. Therapeutic implications of these findings are discussed.