gms | German Medical Science

60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit den Benelux-Ländern und Bulgarien

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

24. - 27.05.2009, Münster

Histological changes after experimental SAH in rats: correlation with perfusion weighted imaging, vasospasm and clinical assessment

Meeting Abstract

  • E. Güresir - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • A. Raabe - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • P. Raab - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • S. Dias - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • V. Seifert - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • H. Vatter - Klinik für Neurochirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main

Deutsche Gesellschaft für Neurochirurgie. 60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit den Benelux-Ländern und Bulgarien. Münster, 24.-27.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. DocP05-07

DOI: 10.3205/09dgnc299, URN: urn:nbn:de:0183-09dgnc2997

Veröffentlicht: 20. Mai 2009

© 2009 Güresir et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: The time course of cerebral vasospasm and clinical changes in the rat double-SAH model are well understood. However, data of histological changes are still missing. Our objective was to determine histological changes in correlation to clinical and perfusion weighted imaging (PWI) changes in the double-SAH model of the rat.

Methods: Cerebral vasospasm (CVS) was induced by injection of 0.25ml autologous blood twice in the cisterna magna of 8 Sprague-Dawley rats. The animals were examined on days 2, 3, 4 and 5 and compared to the sham-operated control group (n=8). The functional deficits were graded between 0 and 3. PWI with 3 Tesla magnetic resonance (MR) tomography was performed on day 5 to assess CBF. The brains were fixed, stained and evaluated for histological changes.

Results: Neurological state was significantly worsened on days 2 and 5 in rats with SAH (medians grade 2 and 3). Sham-operated animals were grade 0 at any time. The relative CBF/muscle blood ratio in rats with SAH was 8.7±0.7 versus 4.3±0.3 (sham, p<0.001). Basilar artery (BA) diameter was 79±5µm (SAH) vs. 143±3 µm (sham, p<0.001). Neuronal cell count in the hippocampal areas CA1–CA4 was significantly reduced by SAH on day 5 (p<0.001). In CA1 cell count was reduced from 78±6 to 49±6, in CA2 from 68±5 to 44±8, in CA3 from 54±6 to 32±7, in CA4 from 51±6 to 32±9.

Conclusions: SAH in the rat double-SAH model leads to vasospasm proven by PWI, neurological worsening and reduced BA diameter. Correlated to these findings, we for the first time demonstrate a reproducible reduction of neuronal cell count in all hippocampal areas. Therefore, the double-SAH model of the rat seems to be an ideal model to investigate cerebral vasospasm and the resulting cerebral lesions.