gms | German Medical Science

60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit den Benelux-Ländern und Bulgarien

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

24. - 27.05.2009, Münster

Free caspase activity – a prognostic indicator in patients suffering from subarachnoid haemorrhage?

Meeting Abstract

  • H.J. Heiroth - Klinik für Neurochirurgie, Klinikum der Heinrich-Heine-Universität Düsseldorf
  • J. Höllerich - Klinik für Neurologie, Klinikum der Heinrich-Heine Universität Düsseldorf
  • P. Albrecht - Klinik für Neurologie, Klinikum der Heinrich-Heine Universität Düsseldorf
  • A. Methner - Klinik für Neurologie, Klinikum der Heinrich-Heine Universität Düsseldorf
  • H.-J. Steiger - Klinik für Neurochirurgie, Klinikum der Heinrich-Heine-Universität Düsseldorf
  • D. Hänggi - Klinik für Neurochirurgie, Klinikum der Heinrich-Heine-Universität Düsseldorf

Deutsche Gesellschaft für Neurochirurgie. 60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit den Benelux-Ländern und Bulgarien. Münster, 24.-27.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. DocDI.03-06

DOI: 10.3205/09dgnc123, URN: urn:nbn:de:0183-09dgnc1239

Veröffentlicht: 20. Mai 2009

© 2009 Heiroth et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: The most frequent reason of morbidity and mortality in patients suffering from subarachnoid haemorrhage (SAH) resulting from a ruptured aneurysm is the occurrence of cerebral vasospasm. The early diagnosis to detect cerebral vasospasm is extremely important. The goal of the present study was to analyze the correlation of initial and following free caspase activity with the occurrence of cerebral vasospasm in patients after aneurysmal SAH.

Methods: Cerebrospinal fluid (CSF)-probes, taken from the external ventricular drainage (EVD) was centrifuged and aliquoted. Free caspase activity was measured by the caspase-GLO 3/7-assay (Promega). The clinical status according to the Glasgow-Coma-Scale (GCS) was documented daily. Furthermore vasospasm was detected using transcranial sonography (TCD), perfusion computerized tomography (PCT) as well as cerebral angiography (DSA).

Results: Preliminary results of 61 probes of 8 SAH-patients and five controls led to a significantly higher (P<0.05 two-tailed t-test) activity of free caspase in the first CSF-probe (Mean 1230 U/l, SEM 135.2, n=5) compared to controls (Mean 910.8 U/l, SEM 2.9, n=5). There was no significant correlation between caspase-activity of initial CSF and initial clinical status (GCS and WFNS). However, a negative correlation exists between the progressive clinical status and caspase-activity in CSF (P<0.05 two-tailed t-test), i.e. the lower the GCS the higher the caspase-activity and vice versa.

Conclusions: In this preliminary study free caspase-activity appears to be a measurable factor in CSF after SAH which correlates negatively with the GCS in SAH-patients. Therefore further investigations are necessary to determine the potency of free caspase activity as a surrogate marker for cerebral vasospasm and clinical outcome in patients after SAH.