gms | German Medical Science

60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit den Benelux-Ländern und Bulgarien

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

24. - 27.05.2009, Münster

Effects and mechanisms of Erythropietin on experimental vasospasm in the rat double haemorrhage model

Meeting Abstract

  • H. Vatter - Neurochirurgische Klinik, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • E. Güresir - Neurochirurgische Klinik, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • P. Raab - Institut für Neuroradiologie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • N. Vasiliadis - Neurochirurgische Klinik, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main
  • A. Raabe - Neurochirurgische Klinik, Inselspital, Bern, Schweiz
  • V. Seifert - Neurochirurgische Klinik, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt am Main

Deutsche Gesellschaft für Neurochirurgie. 60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit den Benelux-Ländern und Bulgarien. Münster, 24.-27.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. DocDI.03-01

DOI: 10.3205/09dgnc118, URN: urn:nbn:de:0183-09dgnc1187

Veröffentlicht: 20. Mai 2009

© 2009 Vatter et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Experimental and recently also clinical investigations suggest a beneficial effect of Erythropoietin (EPO) on the consequences of reduced cerebral blood flow (CBF) during cerebral vasospasm (CVS) after subarachnoid haemorrhage (SAH). However, the mechanisms underlying this effect remaining unclear so far. Therefore, the aim of the present investigation was to characterize the neuroprotective and vasoprotective effects of EPO during CVS.

Methods: CVS was induced in male rats by injection of 0.25 ml autologous blood in the cisterna magna on the days 1 and 2 and assessed on day 5 by the reduction of the relative regional CBF by MR perfusion weighted imaging (PWI) and the diameter of the basilar artery (BA) after histological staining. Ischemic cerebral lesions were determined by the count of intact cells in the hippocampus sections CA1 to CA4. Additionally, the animals were neurological graded between 0 and 3. Rats received 2000 iE EPO intravenously after each artificial bleeding (SAH+EPO). They were compared to a control group (SAH without EPO) and a sham operated group (no SAH without EPO). Each group consisted of 8 animals.

Results: Compared to the sham group relative regional CBF was significantly reduced to 49% in the SAH without EPO group and improved to 74% (SAH+EPO). Diameter of the BA was 143±3 μm (sham) and decreased significantly in both SAH groups: 79±5 μm (without EPO), 109±3 μm (SAH+EPO). Accordingly, the median neurological grade was 0 in the sham group, significantly worsened in the SAH without EPO group to 2.5 and improved to 1 in the SAH+EPO group. The cell count per section in the hippocampus was 63±13 (median±SD) in the sham group, significantly reduced to 39±8 (SAH without EPO) and recovered nearly completely in the SAH+EPO group (61±9).

Conclusions: The present data indicate a significant improvement of the impaired CBF during CVS by EPO without complete removal of the BA narrowing. Therefore, EPO may improve CBF mainly by effects on the microcirculation. Furthermore, the significant recovery of the neurological grade and the reduction of neuronal cell lesions suggest an additive neuroprotective effect of EPO.