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59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
3. Joint Meeting mit der Italienischen Gesellschaft für Neurochirurgie (SINch)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

01. - 04.06.2008, Würzburg

Tracking transplanted cells using anorganic or organic dye strategies

Anorganische oder organische Markierungstrategien im Monitoring transplantierter Zellen

Meeting Abstract

  • corresponding author K. Gaber - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • E. Hempel - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • J. Hirrlinger - Interdisziplinäres Zentrum für Klinische Forschung (IZKF), Universitätsklinikum Leipzig
  • J. Meixensberger - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • M. Skardelly - Klinik für Neurochirurgie, Universitätsklinikum Leipzig

Deutsche Gesellschaft für Neurochirurgie. Società Italiana di Neurochirurgia. 59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3. Joint Meeting mit der Italienischen Gesellschaft für Neurochirurgie (SINch). Würzburg, 01.-04.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocP 017

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2008/08dgnc285.shtml

Veröffentlicht: 30. Mai 2008

© 2008 Gaber et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Monitoring the graft is of fundamental importance to understand and estimate the impact of cell replacement in the concept of transplantation as a therapeutic approach for different diseases. To unequivocally identify and track the graft over a longer period in the host tissue the labeling must be stably incorporated by the cells and should not be transferred to endogenous cells. Furthermore cell function should not be compromised by the labeling technique. In dependency of the scientific question for in vivo imaging the usage of long lasting fluorescence dyes is essential.

Methods: In the first and second mode murine fetal neural stem cells (mfNSC) were stained with anorganic non-fluorescence (very small iron oxid particles (VSOPs) and alternatively fluorescence dyes (PKH-26 and TAMRA) in vitro. In a third manner mfNSC were prepared from transgenic mice expressing a cyano fluorescence protein (CFP) under the control of a β-Actin-Chicken-Promotor. Thereafter cells were transplanted after traumatic brain injury using the Controlled Cortical Impact (CCI) model. To different timepoints (3d, 7, 28 and 84) animals were sacrificed and the graft localized and characterized by immunohistochemistry.

Results: Transgenic mfNSC expressing CFP can be prepared and show longlasting fluorescence over many passages and in other aspects no difference to wild type stem cells. Cells can also easily be stained with VSOPs and PKH-26 or TAMRA. Although transfection/staining rates and cell survival a very high (>90%), costaining with immunohistochemical methods is impaired with fluorescence dyes. VSOP can also be used to monitor the graft in MRI but are not useful for microscopic in vivo monitoring due to their non-fluorescence. PKH-26 and Tamra show a high and longlasting fluorescence in vitro as well as in vivo, but fail to show cellular processes and integration in later timepoints, which was possible with the transgenic CFP expressing stem cells.

Conclusions: Anorganic dyes fulfill most requirements for tracking the graft but fail to visualize extensions and by that structural integration because of redeployment of the dye into special intracellular compartments over time. Organic dyes like the cyano fluorescence protein overcome this restriction and are by that the perfect tool for monitoring most transplantation experiments.