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59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
3. Joint Meeting mit der Italienischen Gesellschaft für Neurochirurgie (SINch)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

01. - 04.06.2008, Würzburg

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Comparison between protein expression profiles in human glioma samples and in human glioma cells lines obtained from the low- and high-grade tumor: our preliminary experience

Meeting Abstract

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  • corresponding author F. Odreman - International Centre for Genetic Engineering and Biotechnology, Padriciano, Trieste, Italy
  • G. Triolo - International Centre for Genetic Engineering and Biotechnology, Padriciano, Trieste, Italy
  • A. Vindigni - International Centre for Genetic Engineering and Biotechnology, Padriciano, Trieste, Italy
  • T. Ius - Neurosurgical Unit, Azienda Ospedaliero Universitaria, Udine, Italy
  • M. Vindigni - Neurosurgical Unit, Azienda Ospedaliero Universitaria, Udine, Italy
  • B. Zanotti - Neurosurgical Unit, Azienda Ospedaliero Universitaria, Udine, Italy
  • D. Musiello - C.I.M.E. University of Udine Medical School, Udine, Italy
  • A. P. Beltrami - C.I.M.E. University of Udine Medical School, Udine, Italy
  • D. Cesselli - C.I.M.E. University of Udine Medical School, Udine, Italy
  • C. A. Beltrami - C.I.M.E. University of Udine Medical School, Udine, Italy
  • M. Skrap - Neurosurgical Unit, Azienda Ospedaliero Universitaria, Udine, Italy

Deutsche Gesellschaft für Neurochirurgie. Società Italiana di Neurochirurgia. 59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3. Joint Meeting mit der Italienischen Gesellschaft für Neurochirurgie (SINch). Würzburg, 01.-04.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocP 003

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2008/08dgnc271.shtml

Veröffentlicht: 30. Mai 2008

© 2008 Odreman et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

In our previous studies, we compared the protein expression profiles of low- and high-grade human brain astrocytoma tissues by two-dimensional gel electrophoresis. In this study, we extended the same analysis to cell lines obtained from the low- and high-grade tumor samples.

For this reason, main objectives of our study were: 1. To optimize a method to isolate and expand in vitro cancer stem cell (CSC) population from low-grade and high-grade human brain astrocytomas (named, respectively, l-CSCs and h-CSCs); 2. To demonstrate in vitro l-CSC and h-CSC stemness and tumorigenicity; 3. To compare the protein expression profiles of l-CSCs and hCSCs.

Low- (n=17) and high- (n=19) grade astrocytomas were obtained by surgical resection at the Neurosurgical Unit of the Azienda Ospedaliero-Universitaria of Udine. Neoplastic tissues were mechanically-enzimatically dissociated and the single cell suspension cultured in a medium selective for the growth of Multipotent Adult Stem Cells. Tumorigenicity of expanded cell lines was confirmed by the demonstration of anchorage independence growth (soft agar assay) and loss of contact inhibition (sphere formation assay, transformation foci assay, and growth curve analysis).

The proteomic studies performed to compare h-CSCs and l-CSCs allowed us to identify a number of proteins that are more abundant either in the low- or in the high-grade tumors and that were not detected in the previous study using the brain tissues. Moreover, we also used a staining procedure selective for phosphorylated proteins to highlight eventual differences in the phosphoproteome of the tumors of different grade.