Artikel
The short-term influence of neural stem cell transplantation upon the regional energy metabolism following experimental spinal cord trauma
Der kurzfristige Einfluss von transplantierten neuralen Stammzellen auf den regionalen Energiestoffwechsel nach experimentellem Rückenmarktrauma
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Veröffentlicht: | 11. April 2007 |
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Gliederung
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Objective: The transplantion of neural stem cells (NSC) in trauma models has been shown to decrease secondary tissue damage and to rescue damaged host neurons. While one possible mechanism may be trophic factor production, we explored the hypothesis that it might also be due to restoration of the metabolic capacity of the impaired spinal cord tissue after trauma.
Methods: 63 male C57BL/6J mice (8-12 weeks of age; 19-23g) were deeply anaesthetized and a laminectomy was performed at the T7 – T9 level. A mouse spinal cord compression device was used for complete compression injury except in the sham control group. Except for the animals of the trauma only control groups, 2 ul of buffer solution with and without NSC were injected into the center of the lesion. Animals were sacrified 4h and 24h postinjury and the vertebral columns were deeply frozen. The spinal cords were dissected free and regional metabolic parameters (glucose, ATP and lactate) were measured using a bioluminiscence technique.
Results: At the lesion site, all measured parameters broke down and NSC transplantation did not induce any detectable change. In adjacent regions, there was an increased glucose and ATP concentrations with NSC cell injection.
Conclusions: Our results indicate that the regional metabolism adjacent to a spinal cord trauma is positively influenced by the presence of transplanted NSC.