gms | German Medical Science

57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

11. bis 14.05.2006, Essen

Modified calcium accumulation after controlled cortical impact under Cyclosporin A treatment. A 45Ca autoradiographic study

Alteration des posttraumatischen Kalziumstoffwechsels unter Cyclosporin A im Rattenmodell ("Controlled Cortical Impact")

Meeting Abstract

  • corresponding author M.J. Mirzayan - Department of Neurosurgery, Medical School Hannover
  • S. Uhde - Department of Neurosurgery, International Neuroscience Institute Hannover
  • P. Klinge - Department of Neurosurgery, International Neuroscience Institute Hannover
  • M. Samii - Department of Neurosurgery, International Neuroscience Institute Hannover
  • T. Brinker - Department of Neurosurgery, International Neuroscience Institute Hannover
  • Z. Korkmaz - Department of Nuclearmedicine, Medical School Hannover
  • G.J. Meyer - Department of Nuclearmedicine, Medical School Hannover
  • J.K. Krauss - Department of Neurosurgery, Medical School Hannover
  • W.H. Knapp - Department of Nuclearmedicine, Medical School Hannover
  • A.C. Stan - Department of Pathology, Medical School Hannover
  • A. Samii - Department of Neurosurgery, International Neuroscience Institute Hannover

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. Essen, 11.-14.05.2006. Düsseldorf, Köln: German Medical Science; 2006. DocP 03.34

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2006/06dgnc251.shtml

Veröffentlicht: 8. Mai 2006

© 2006 Mirzayan et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Objective: As a neuroprotective drug Cyclosporin A has been subject of multiple experimental studies in traumatic brain injury research. It is well known that Cyclosporin A inhibits Calcium induced mitochondrial permeability transition (mPT) and the subsequent release of proapoptotic proteins. We investigated the calcium homeostasis after CCI and Cyclosporin A treatment.

Methods: Sprague-Dawley male rats (n=36) with a mean weight of 330g (280-350 g) were anesthetized with Isofluran. The anesthetized animals (n=24) were subjected to a CCI over the left parieto-temporal cortex using round-tip impounder with a 5 mm diameter at a velocity of 3.7 m/sec and a penetration depth of 2 mm. Cyclosporin A (n=12) or vehicle (n=12) was administered 15 min post injury with a subsequent i.p. injection 24 h post-injury. One group of animals (n=12) underwent anesthesia and craniotomy without CCI. At 43 hours after injury 45Ca suspended in physiological saline solution was injected in the left femoral vein. Five hours after isotope administration animals were sacrified and the brain was quickly removed and placed in powdered dry ice. Coronal plane sections (20µm thick) taken every 400µm from the frontal cortex through the occipital cortex were exposed to Cyclotron films for 14 days. Relative optical density was utilized to provide a relative measure of 45Ca accumulation within seven different structures.

Results: The difference of calcium accumulation (measured by relative optical density) between the Cyclosporin A group and vehicle treated animals ranged between 30 and 70% in the following structures: caudate putamen, antero-medial thalamus, postero-medial thalamus, temporal cortex, occipital cortex, CA 1 and CA 3 (p<0.05). Only the frontal cortex showed no significant changes of the 45Ca accumulation between the different groups.

Conclusions: Posttraumatic calcium accumulation is modified under Cyclosporin A. There is a significant difference of calcium accumulation between the treatment groups.