gms | German Medical Science

57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

11. bis 14.05.2006, Essen

Induction of differentiation of murine neural stem cells by confrontation with highly regenerative blastema tissue of the axolotl salamander

Induzierung von Differenzierungsmechanismen von murinen neuralen Stammzellen durch Konfrontation von regenerierendem Blastemgewebe des Axolotlsalamanders

Meeting Abstract

  • corresponding author M. Kirsch - Klinik und Poliklinik für Neurochirurgie, Carl Gustav Carus Universitätsklinikum, Technische Universität Dresden
  • M. Niesche - Klinik und Poliklinik für Neurochirurgie, Carl Gustav Carus Universitätsklinikum, Technische Universität Dresden
  • B. Kilian - Klinik und Poliklinik für Neurochirurgie, Carl Gustav Carus Universitätsklinikum, Technische Universität Dresden
  • G. Schackert - Klinik und Poliklinik für Neurochirurgie, Carl Gustav Carus Universitätsklinikum, Technische Universität Dresden
  • H.K. Schackert - Chirurgische Forschung, Carl Gustav Carus Universitätsklinikum, Technische Universität Dresden
  • E. Tanaka - Max Planck Institute for Molecular Cell Biology and Genetics, Dresden

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. Essen, 11.-14.05.2006. Düsseldorf, Köln: German Medical Science; 2006. DocSO.03.08

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2006/06dgnc180.shtml

Veröffentlicht: 8. Mai 2006

© 2006 Kirsch et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Caudate amphibians such as the Axolotl salamander (Ambystoma mexicanum) regenerate an amputed tail including a fully functional spinal cord. After amputation, a multipotent array of proliferating and differentiating cells termed blastema develops which gives rise to all cell types of the regenerating tail and spinal cord. This model represents an opportunity to understand what factors are required to induce a neural stem cell to undergo productive regeneration. The purpose of our study was to investigate the differentiation potential of murine neural stem cells (NSC) in response to intrinsic signalling of the blastema.

Methods: Primary murine embryonic cerebellar neural stem cells were grown as neurospheres in neural stem cell medium. Blastema were obtained 5-7 days after amputation of the axolotl tail. After removal of the overlaying skin, the blastema was removed under a dissecting microscope. Murine neurospheres were juxtaposed with blastema tissue in vitro. The invasion of NSC into the blastema was analyzed by confocal microscopy. Differentiation and proliferation of NSC was analysed using specific antibodies against Nestin, NeuN, β3-Tubulin, GFAP, Ki67, PCNA, BrdU, CNPase, and GalC.

Results: In vitro conditions were optimized to allow both NSC and blastema cells to survive and proliferate. Within 3 days NSC were able to invade into the regenerating blastema. Depending on their location in relation to the blastema, proliferation occurred mainly in the center of the neurosphere contrasting to isolated neurospheres which show predominant proliferation in the periphery. Upon confrontation, a strong differentiation response was seen showing an increase of oligodendroglial and neuronal cells but a decrease of precursor and astrocytic cells as compared to conventional in vitro induction of differentiation via growth factor withdrawal.

Conclusions: Murine NSC responded to a regenerative environment such as axolotl blastema tissue and revealed a higher differentiation potential compared to standard induction of differentiation. Further studies are aiming to identify the factors responsible for the higher regenerative potential induced by blastema tissue.