gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Functional and phenotypical characterisation of endothelial cells derived from gliomas of different grades

Funktionelle und phänotypische Charakterisierung endothelialer Zellen aus Gliomen verschiedener Grade

Meeting Abstract

  • corresponding author S. Grau - Neurochirurgische Klinik, Klinikum Großhadern, LMU München
  • S. Miebach - Neurochirurgische Klinik, Klinikum Großhadern, LMU München
  • O. Schnell - Neurochirurgische Klinik, Klinikum Großhadern, LMU München
  • C. Schichor - Neurochirurgische Klinik, Klinikum Großhadern, LMU München
  • J.-C. Tonn - Neurochirurgische Klinik, Klinikum Großhadern, LMU München
  • R. Goldbrunner - Neurochirurgische Klinik, Klinikum Großhadern, LMU München

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP178

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2005/05dgnc0446.shtml

Veröffentlicht: 4. Mai 2005

© 2005 Grau et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

The ability to induce neo-angiogenesis is a key feature in glioma grading. Investigations of endothelial cells (EC) from various organs revealed significant differences between the populations depending on organ and dignity of the tissue of origin. For assessment of tumour induced angiogenesis use of organ- and tissue-specific EC appears mandatory.

Methods

EC were isolated from 22 low grade astrocytomas (LGEC), 11 malignant gliomas (HGEC) and 7 normal brain specimen (obtained from epilepsy surgery procedures). Isolation was performed by combining density gradient separation with magnetic bead sorting. Characterisation of EC was performed by measuring the expression of EC-specific cell surface antigens. On mRNA level expression of MMP 2, 3, 7, 9 and TIMP 1, 2 and 3 was measured by real-time rt-PCR. Activity of MMP 2 and 9 was assessed by zymography. Functional ability of the cells was evaluated by means of proliferation, tube-formation and co-culture.

Results

A reproducible method for isolating EC from brain tumours could be established. There was no correlation seen between antigen expression pattern and cell morphology. Expression of EC specific antigens was strongly influenced by cell culture conditions, surface antigens like wWF were only expressed in primary cultures while CD31 and VE-Cadherin could be shown present up to passage five. Regarding proliferation there were major differences between EC subpopulations with HGEC showing a four-fold higher proliferation rate than LGEC and NBEC. In MMP expression mRNA for MMP 2, 3, and 7 was found in all EC; MMP 9, however, was exclusively expressed in HGEC. None of the EC isolated could form tubes in vitro, even in presence of various stimulating factors.

Conclusions

Due to rapid changes in EC antigen expression the use of freshly isolated cells in very low passages should be obligatory. Some first functional assessments show clear differences between NBEC, LGEC and HGEC pointing towards a different biological behaviour among these cell populations. Thus, for investigating glioma angiogenesis the use of glioma specific EC is mandatory. The differences between EC shown above may reveal a contribution of endothelial cells to the malignant phenotype of the tumour.