gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Cellular Plasticity of endogenous neural precursors responding to glioblastomas

Glioblastom induzierte zelluläre Plastizität endogen neuronaler Stammzellen

Meeting Abstract

  • corresponding author M. Synowitz - Department of Neurosurgery, Helios Hospital Berlin; Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin
  • R. Glass - Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin
  • G. Kronberg - Volkswagen Stiftung Research Group, Deparment of Experimental Neurology, Charité University Hospital, Humboldt University, Berlin
  • J. H. Waelzlein - Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin
  • D. Markovic - Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin
  • L. P. Wang - Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin
  • D. Gast - Volkswagen Stiftung Research Group, Deparment of Experimental Neurology, Charité University Hospital, Humboldt University, Berlin
  • J. Kiwit - Department of Neurosurgery, Helios Hospital Berlin
  • G. Kempermann - Volkswagen Stiftung Research Group, Deparment of Experimental Neurology, Charité University Hospital, Humboldt University, Berlin
  • H. Kettenmann - Cellular Neuroscience Group, Max Delbrück Centre for Molecular Medicine (MDC), Berlin

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP168

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2005/05dgnc0436.shtml

Veröffentlicht: 4. Mai 2005

© 2005 Synowitz et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

Nestin-positive cells are found within and around human glioblastomas and were thought to represent dedifferentiating cells originating from these tumors. We now demonstrate that these nestin expressing cells are neural progenitors cells from the subventricular zone, which migrate to the tumor and subsequently differentiate.

Methods

G261 glioblastoma cells stably expressing DsRed were injected into striatum of transgenic mice expressing green fluorescent protein under the nestin promoter. This mouse model allowed us to distinguish nestin-positive cells of the brain parenchyma from those generated by the tumor. Immunofluorescent triple labeling was carried out on 40-μm-free-floating sections using a spectral confocal microscope.

Results

14 days after injection nestin-positive cells in brains of 25 day old mice surrounded the glioblastoma with several cellular layers. The first nestin-positive cells were found at the tumor 4 days after injection, while the highest density was reached after 14 days. Tumors in young animals attracted more nestin-positive cells since the presence of neural progenitors declines with increasing age (comparing animals 100, 180 and 360 days of age). Nestin-positive cells co-expressed markers for progenitor cells (Musashi, TUC-4), immature neurons (doublecortin) and glial cells (NG-2,GFAP) and proliferated (Ki67). Patch-clamp studies indicated that cells expressed a current profile of immature glial cells. Markers for mature astrocytes and neurons (S-100β and β-tubulin) did not label nestin-positive cells. The progenitors originated from the subventricular zone, since intraventricular administration of DiI prior to tumour inoculation resulted in DiI stained nestin-positive cells surrounding the tumour. BrdU labelling indicated that the nestin-positive cells had previously divided. Moreover, in explant cultures from the subventricular zone precursor cells showed extensive tropism for glioblastoma aggregates.

Conclusions

Our data indicate that glioblastomas attract endogenous neural progenitor cells from the subventricular zone, which expressed markers of early, non-committed and of committed precursors.