gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

The mitochondrial ribosome function protein MRP-L32 is associated with malignancy of human glial tumors

Das mitochondriale Ribosom-Funktionsprotein MRP-L32 ist assoziiert mit der Malignität glialer Tumoren

Meeting Abstract

  • corresponding author K. D. Geiger - Institute of Neuropathology, University Leipzig, Institute of Pathology, University Dresden
  • W. Krupp - Department of Neurosurgery, University Leipzig
  • A. Goldammer - Department of Neurosurgery, University Leipzig
  • S. Pregizer - Institute of Neuropathology, University Leipzig
  • R. Schober - Institute of Neuropathology, University Leipzig
  • J. Meixensberger - Department of Neurosurgery, University Leipzig

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP162

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2005/05dgnc0430.shtml

Veröffentlicht: 4. Mai 2005

© 2005 Geiger et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

Mitochondria play an important role in therapeutically induced apoptosis of tumor cells. To analyze the prognostic role of mitochondrial morphology, we studied gliomas of varying dignity with respect to possible prognostic prediction, using MRP-L32, a phosphorylation associated mitochondrially encoded ribosomal function protein.

Methods

We used a novel antibody to MRP-L32 with the indirect peroxidase technique on paraffin-embedded tissue of normal human brain and 136 glial tumors of varying dignity WHO grade I-IV. For comparison, localization of the protein was studied in primary cell cultures of glioblastoma (n=6) using oregon green or Cy3-labeled secondary antibody. In addition we studied proliferation (MIB-1) and MTCO2 for mitochondrial numbers. Immunoreaction (IR) was evaluated using a semiquantative scoring system and statistical evaluation with the chi square test.

Results

We found a low constitutive expression of MRP-L32 in normal human brain, mostly in astrocytes with emphasis on subependymal and perivascular astrocytes. There was no increased IR in benign gliomas. In malignant gliomas, approximately 40% of the cases showed strongly increased IR. We found a signifiant association with malignancy, though without difference between grades III and IV tumors There was no correlation with proliferation within glioblastomas or with mitochondrial frequencies.

Conclusions

The observed results suggest a dichotomy of malignant tumors with regard to the expression of MRP-L32, which is not dependent on mitochondrial frequencies. It still has to be established whether this is due to a genetic or a pathway - related effect. Results further suggest that the protein may provide an identification of different subtypes of malignant gliomas, sensitive to different treatment protocols.