Artikel
Predictive value of p(ti)O2
and EEG monitoring as outcome measure in a rodent mass lesion model
Prediktiver Wert des p(ti)O2
und EEG Monitorings für das Outcome nach fokaler Hirnläsion bei der Ratte
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Autoren
Veröffentlicht: | 4. Mai 2005 |
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Gliederung
Text
Objective
Predictive value of brain p(ti)O2 and EEG monitoring on a 2 week assessment of behavorial deficits after a focal mass lesion model in rodents.
Methods
25 Spraque Dawley rats were included (224±17g) in the study. Complete monitoring was able in 18/25 and long-term neurobehavioral testing in 13/18 animals. Baseline measurement (60±3min) of brain partial oxygen pressure [p(ti)O2] and EEG (visual inspection scale, 1=isoelectric, 5=good) was followed by induction of an epidural focal mass lesion with a balloon. The endpoint of balloon inflation was defined by flattening of the EEG. The mass lesion was prolonged over 60 min. After reperfusion animals were followed over 61±3 min before animals were extubated and brought back to their cages. Posttraumatic mortality was evaluated and neurobehavior assessed over 14 days [beam balance (BB); beam walking (BW); open field (OF); postural reflex (PR); Morris water maze (MWM). The lesion was also evaluated by MRI and histology.
Results
Intraischemic p(ti)O2 values at the end of prolonged brain compression in deceased animals were lower (2.8±2.5mmHg) compared to surviving animals (10±7mmHg, p=0.016) and corresponded with EEG inpections scores in both groups (p=0.03). After 60 min of reperfusion p(ti)O2 did not differ between deceased and surviving animals (p=0.821, 23±6 mmHg versus 24±8 mmHg) while EEG activity was significantly depressed in deceased compared to surviving animals (p=0.003; 1.8±0.8/ 4.4±1). In animals which survived p(ti)O2 and EEG scoring values at 60 min of prolonged balloon compression and after reperfusion did not correlate with individual neurobehavior 14 days after the trauma.
Conclusions
Intraischemic p(ti)O2 and intraischemic and acute postischemic EEG monitoring predict fatal outcome. Long-term neurobehavior did not correlate with individual results during neuromonitoring.