gms | German Medical Science

55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

Serotonin 5-HT2A receptors are regulators for glial tumor growth

Die Bedeutung von 5-HT2A-Rezeptoren für das biologische Verhalten glialer Hirntumore

Meeting Abstract

  • corresponding author Michael Synowitz - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin; AG Zelluläre Neurowissenschaften, MDC Berlin-Buch, Robert Rössle Straße 10, 13092 Berlin
  • M. Glaß - AG Zelluläre Neurowissenschaften, MDC Berlin-Buch, Robert Rössle Straße 10, 13092 Berlin
  • D. Markovic - AG Zelluläre Neurowissenschaften, MDC Berlin-Buch, Robert Rössle Straße 10, 13092 Berlin
  • H. Kettenmann - AG Zelluläre Neurowissenschaften, MDC Berlin-Buch, Robert Rössle Straße 10, 13092 Berlin
  • J. Kiwit - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocP 05.48

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2004/04dgnc0331.shtml

Veröffentlicht: 23. April 2004

© 2004 Synowitz et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

It is known that glutamate release from glial tumor cells promotes growth of malignant glioma. It is also accepted that activation of 5-hydroxytryptamine2A (5-HT2A) receptors on cultured glia stimulates glutamate efflux. In this study we have addressed the question whether 5-HT2A receptors modulates growth of glial tumor cells.

Methods

Expression of serotonin 5-hydroxytryptamine2A (5-HT2A) receptors were examined by Western blot analysis, immunocytochemistry, and immunofluorescent double staining in mice glial tumor cells, primary cultures of mice neuroglia and human tumor material. Organotypical brain slice cultures were prepared from 16-day-old C57BL/6-mices. Coronal slices of 250µm thickness were cut and placed in wells filled with standard culture medium. G261 glioma cells were co-transfected with enhanced green fluorescent protein (pegfp-N1) using LipofectAmin. Thousand cells were solved in 0.1µl and injected into the basal ganglia of each slice via a stereotactic device. Specific 5-HT2A agonists and antagonists were added daily. Single tumor cell invasion and proliferation was observed by confocal laser scanning microscopy from day 1 to 7 and analyzed using software ImagePro.

Results

Neuroglia and glial tumor express 5-HT2A receptor in all tested species. Interestingly microglia is the major source of 5-HT2A expression. Activation of the 5-HT2A receptor using specific agonists showed a significant increase on tumor invasion and migration. Inhibition of the 5-HT2A receptor using specific antagonists inhibit invasion as well as proliferation over time and from day three compared to the control group.

Conclusions

Our results support the idea that the glutamate release from glial tumor cells, which promotes growth of malignant glioma, is mediated by serotonin 5-HT2A receptors. Further experiments have to reveal the role of 5-HT2A receptors on microglia and the importance for microglia – tumor interaction.