gms | German Medical Science

55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

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Comparison of S100B serum levels versus cerebral levels assessed by MR Spectroscopy after fluid percussion injury

Vergleich von S100B Serum versus zerebrale Konzentration, erfasst mittels MR-Spektroskopie nach experimentellem Schädelhirntrauma

Meeting Abstract

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  • corresponding author Andrea Kleindienst - Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA
  • C. Mueller - Institute of Laboratory Medicine and Pathobiochemistry, Charite, Humboldt University Berlin, Berlin
  • C. M. Tolias - Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA
  • F. D. Corwin - Department of Radiology, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA
  • P. Fatouros - Department of Radiology, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA
  • A. Marmarou - Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA
  • M. R. Bullock - Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond /USA

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocP 01.5

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2004/04dgnc0288.shtml

Veröffentlicht: 23. April 2004

© 2004 Kleindienst et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

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Objective

S100B is released by astrocytes into the extracellular fluid after a variety of brain insults, and CSF and serum S100B levels have been correlated to outcome after traumatic brain injury (TBI). As no data exist about the temporal profile of S100B cerebral levels, and their correlation to serum levels, we examined whether MR spectroscopy (MRS) is capable of depicting cerebral S100B levels after TBI.

Methods

At different time points following lateral fluid percussion or sham injury in male Sprague Dawley rats, blood samples were analysed for S100B using a chemiluminescence immunoassay (LIAISON® Sangtec®, ByK-Sangtec Diagnostica, Dietzenbach, Germany). At the same time points, MRS was performed on a 2.35 T magnet (gradient strength 25G/cm; Bruker Instr.) using a water suppressed SE voxel localization technique (5x5x5mm3 voxel, TR/TE 3000/30ms, 256aq). Areas under the resultant peaks (EUC) were manually determined, and normalized to NAA.

Results

S100B serum levels in sham animals were below 0.02 ng/ml. After injury, S100B serum levels increased up to 0.47±0.14 ng/ml at 3 hr, subsequently returning to normal levels. In sham animals MRS showed a S100B specific peak at 4.5ppm with a EUC of 0.02. After injury, the EUC increased to 0.30 at 3 hr, and up to 0.92 on day 5 post-injury.

Conclusions

S100B serum levels were increased for only a very short period after injury. On the other hand, MRS demonstrated increased cerebral S100B levels up to day 5 after experimental TBI. While experimental data suggest that S100B is actively released by astrocytes after brain insults, MRS may offer a tool to identify increased cerebral S100B levels in patients after TBI. Further studies are needed to examine the contribution of this cerebral release to recovery after TBI.