gms | German Medical Science

55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

The impact of brain spatula on the cell integrity of the retracted brain tissue in a porcine model: A feasibility study

Tierexperimentelle Studie zum Einfluss von Hirnspateln auf die Zellintegrität des retrahierten Hirngewebes: eine Machbarkeitsstudie

Meeting Abstract

  • corresponding author Ruth Thiex - Department of Neurosurgery, Aachen University, Aachen
  • F. J. Hans - Department of Neurosurgery, Aachen University, Aachen
  • T. Krings - Department of Neuroradiology, Aachen University, Aachen
  • B. Sellhaus - Department of Neuropathology, Aachen University, Aachen
  • K. Scherer - Institute of Laboratory Animal Sciences, Aachen University, Aachen
  • A. Thron - Department of Neuroradiology, Aachen University, Aachen
  • J. M. Gilsbach - Department of Neurosurgery, Aachen University, Aachen

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocDI.04.09

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2004/04dgnc0187.shtml

Veröffentlicht: 23. April 2004

© 2004 Thiex et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

We describe a porcine brain injury model following brain retraction by brain spatula with regard to experimental setup and biomechanical aspect. The aim was to identify the primary and secondary pathological and functional sequelae associated with brain cortical contusions and perifocal edema following brain retraction.

Methods

In 11 anaesthetized male pigs, the right frontal brain was retracted in the interhemispheric cleft by a brain spatulum with various pressures applied by the gravitation force of weights from 10 to 70 g for the duration of 30 minutes. The integrity of the retracted brain tissue was monitored for changes in intracranial pressure and in perfusion of the cortex using a Laser Doppler Perfusion Imager (LDPI, Lawrenz). To evaluate the extent of edema and cortical contusions MRI was performed 30 minutes and 72 hours after brain retraction. The resulting ischemia and edema were histopathologically assessed using Luxol Fast Blue and Cresylviolet as well as Fluoro-Jade-B staining for neuronal damage.

Results

Following this protocol, a retraction pressure applied by 10 and 20 g weights (n=5) over a duration of 30 minutes caused a mean rise of ICP to 9.6±3.4 mmHg, a decrement in mean cortical perfusion from 754.5±22.1 PU/cm2 to 659.3±131.5 PU/cm2. Retracting the brain with a weight of 30-70 g (n=6), the mean ICP increased to 22.0±4.3 mm Hg and caused the cortical perfusion to drop from a mean of 651.6±99.7 PU/cm2 to 437.4±140.1 PU/cm2 corresponding to a substantial edema on FLAIR images and irreversible neuronal damage on Fluoro-Jade-B-staining.

Conclusions

The cortical impact caused by the retracting brain spatulum was found to be critical at weights beyond 20 g over a period of 30 minutes resulting in a significant decrement in cortical perfusion and irreversible neuronal damage.