gms | German Medical Science

128. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

03.05. - 06.05.2011, München

Use of vascular resident endothelial progenitor cells for scaffold based dermal tissue engineering

Meeting Abstract

  • Ziyang Zhang - Technische Universität München, Deptartment of Plastic and Hand Surgery, Klinikum recht der Isar, Munich
  • U. Hopfner - Dept. Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich
  • Y. Harder - Dept. Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, München
  • A. Reckhenrich - Dept. Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich
  • M. Kremer - University of Lübeck, Department of Plastic Surgery and Hand Surgery, Lübeck
  • N. Lund - University Hospital Hamburg Eppendorf, Department of Experimental Cardiology, Hamburg
  • W. Ito - Academic Teaching Hospital, University of Ulm, Cardiovascular Center Oberallgaeu, Immenstadt
  • J. Lohmeyer - Dept. Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich
  • H.-G. Machens - Klinikum rechts der Isar, Technische Universität München, München
  • J.T. Egaña - Dept. Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich

Deutsche Gesellschaft für Chirurgie. 128. Kongress der Deutschen Gesellschaft für Chirurgie. München, 03.-06.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgch382

DOI: 10.3205/11dgch382, URN: urn:nbn:de:0183-11dgch3824

Veröffentlicht: 20. Mai 2011

© 2011 Zhang et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Increasing evidences suggest that vascular resident endothelial progenitor cells (VR-EPCs) are present in vessel walls, playing an important role in postnatal neovascularization. However, their structural and functional characteristics still remain unclear. In this work we isolated and characterize VR-EPCs in vitro, evaluating their regenerative potential in vivo.

Materials and methods: VR-EPCs were isolated from myocardial tissue in rats and characterized in vitro with regard to morphology, cell surface markers and angiogenic response. Thereafter, cells were seeded in a collagen scaffold and their regenerative potential was evaluated in vivo in a full thickness skin defect model.

Results: Isolated cells share several features with EPC described for other tissues. Amongst others, VR-EPCs expressed CD34, and CD133 and could develop capillary like structures in vitro. After seeding VR-EPCs in a collagen scaffolds, cells were homogeneously distributed, forming focal adhesions within the scaffold which was confirmed by confocal microscope. Metabolic assays showed that cells could proliferate in vitro for at least 2 weeks in the scaffold (P<0.01). In vivo, we observed by transillumination/digital segmentation (P<0.001) and by histology assays (P<0.05) that the presence of these cells enhanced dermal vascularization compared to control scaffolds. In order to explore the possible mechanisms behind the improvement in tissue regeneration, in vitro analysis was performed and showed that isolated cells secreted several pro-angiogenic molecules including VEGF and PDGF. Moreover, the migration capacity of VR-EPCs (P<0.001) and the formation of capillary like structures (P<0.01) were both improved under endothelial culture conditions (Figure 1 [Fig. 1]).

Conclusion: This work indicates that a highly clonogenic VR-EPCs population that exhibits considerable regenerative capacities can be established in vitro. Accordingly, these cells could constitute a new therapeutic approach to induce vascular regeneration in dermal scaffolds.